This is the Process of taking all the individual client’s sample’s necessary tangible information regarding a sample being received not limited to patient name, Date of Birth, specimen type, possible diagnosis, etc. and transferring it to an alphanumeric combination eliminating information that could potentially be used to expose any sensitive patient data (in compliance with HIPPA).
2) Grossing – Dissection of tissue sample in a ventilation hood. Once sample is dissected, it is then placed in a cassette (with or without sponges or tissue paper) for processing.
3) Processing – Tissue then goes through a series of steps: a)Dehydration – Removal of all water present in the tissue sample, usually done with alcohol. b) Clearing – The removal of alcohol from the tissue. This reagent must be miscible in both alcohol and paraffin wax. c)Infiltration – Replacing the clearing agent with a support medium such as paraffin wax (or other infiltration mediums) throughout the tissue sample. Support medium is usually the same as embedding medium.
4) Embedding – Also known as Casting or blocking; This is the process of allowing the infiltration medium to encase the tissue, then allowing the medium to solidify.
5) Microtomy – Cutting the tissue into sections between 4-10 (on average) microns, forming a ribbon and laying it on a floatation bath (water bath). The sections are then picked up on slides, air-dried or dried in a convection oven, and they go to the stainer.
6) Staining – H&E standard hematoxylin and eosin staining.
7) Coverslip – using a toluene based mounting medium and slides are using a #1 thick coverslips.